Batch Fabrication of Shape- Controlled Microstructures for 3D Cell Assembly


2D Ca-alginate cell-laden gel membranes were electrodeposited onto micro-patterned electrode and further detached from the electrode to achieve miniaturized 3D microcapsules in which cells formed multi-cellular aggregate after cultivation for two weeks. The main advantages of our approach including: 1) Gear-like tissue fabrication with predefined shape 2) High cell density while maintaining the viability 3) Movable structures that can be transported and manipulated.


  • Z.liu, M.Takeuchi, M.Nakajima, T.Fukuda, Y.Hasegawa and Q.Huang : "Batch Fabrication of Microscale Gear-Like Tissue by Alginate-Poly-L-lysine (PLL) Microcapsules System.", IEEE Robotics and Automation Letters, 2377-3766, pp 206 – 212, 2016.
  • Z.liu, M.Takeuchi, M.Nakajima, T.Fukuda, Y.Hasegawa and Q.Huang: "Shape-controlled High Cell-density Microcapsules by Electrodeposition.", Acta Biomaterialia, Vol. 31, No. 3, pp. 750–765, 2016